OrtholugeDB contains Ortholuge-based orthology predictions for completely sequenced bacterial and archaeal genomes. The Ortholuge method improves the specificity of high-throughput orthology prediction. More Information.
OrtholugeDB also contains reciprocal best hit-based ortholog predictions, in-paralog predictions (recently duplicated genes) and proposed ortholog groups.
See some example analyses.
Take one index (reference) genome and look for presence/absence of orthologs in one or more comparison strains.